DEGRADATION OF THIODIGLYCOL, THE HYDROLYSIS PRODUCT OF SULFUR MUSTARD, WITH BACTERIA IMMOBILIZED WITHIN POLY(VINYL) ALCOHOL CRYOGELS

Authors
KIM JW RAININA EI EFREMENKO E ENGLER CR WILD JR
Citation
Jw. Kim et al., DEGRADATION OF THIODIGLYCOL, THE HYDROLYSIS PRODUCT OF SULFUR MUSTARD, WITH BACTERIA IMMOBILIZED WITHIN POLY(VINYL) ALCOHOL CRYOGELS, Biotechnology letters, 19(11), 1997, pp. 1067-1071
Citations number
8
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
19
Issue
11
Year of publication
1997
Pages
1067 - 1071
Database
ISI
SICI code
0141-5492(1997)19:11<1067:DOTTHP>2.0.ZU;2-H
Abstract
Alcaligenes xylosoxidans subsp. xylosoxidans (SH91) capable of biodegr adation of thiodiglycol (TDG) were immobilized in poly(vinyl) alcohol (PVA) cryogels. Cryoimmobilized biocatalyst was formed as spherical gr anules with a diameter of 0.5 mm; the biomass concentration inside the gel matrix was as high as 10% (w/w). The immobilized cells were capab le of rapid degradation of TDG in tap water or potassium phosphate buf fer (100 mM, pH 8.0) containing only (NH4)(2)SO4. The immobilized bioc atalyst did not show any substrate inhibition up to 200 mM TDG, and re tained 100% activity during three months of continuous use in a repeat ed-batch bioreactor.