EFFICIENT PRODUCTION OF RECOMBINANT PROTEIN IN SPODOPTERA-FRUGIPERDA ACNPV SYSTEM UTILIZING SILKWORM HEMOLYMPH

Silkworm hemolymph when added at 5% (v/v) to medium increased the prod uction of recombinant beta-galactosidase in Spodoptera frugipera/Autog rapha californica nuclear polyhedrosis virus (AcNPV) system by 4.5-fol d. Silkworm hemolymph also increased the host cell longevity by two-fo ld. Viability of the host cell was maintained at a constant level for 6 days after baculovirus infection in the medium containing 5% silkwor m hemolymph, while host cells began to die 3 days after infection in t he medium without hemolymph.