SPECTROSCOPIC STUDIES TO EVALUATE THE INT ERACTIONS BETWEEN ACRIDINE-ORANGE AND ALCOHOLS

The association states of Acridine Orange (AO) in four kinds of ethano l solutions were studied by absorption spectra. The association state of AO would change with different concentration. A peak assigned to th e AO monomer was appeared when the concentration is 4x10(-5) mol dm(-3 ). The AO dimer was also characterized by their difference spectra. Th e interaction between AO monomer and ethanol molecule was examined by the use of a simplified McRae's equation on the maximum absorption pea k positions. The analyses showed that the orientation force between AO and ethanol is 57.6 cal mol(-1), the sum of the dispersion and induct ion forces is 219 cal mol(-1), and the hydrogen bonding force is 125 c al mol(-1).