ROOM-TEMPERATURE STORAGE AND CRYOPRESERVATION OF CANINE PLATELET CONCENTRATES

Citation
K. Allyson et al., ROOM-TEMPERATURE STORAGE AND CRYOPRESERVATION OF CANINE PLATELET CONCENTRATES, American journal of veterinary research, 58(11), 1997, pp. 1338-1347
Citations number
51
Categorie Soggetti
Veterinary Sciences
ISSN journal
00029645
Volume
58
Issue
11
Year of publication
1997
Pages
1338 - 1347
Database
ISI
SICI code
0002-9645(1997)58:11<1338:RSACOC>2.0.ZU;2-F
Abstract
Objective-To determine whether in vitro viability and function, and mi crobiological sterility, of canine platelet concentrates (PC) could be retained during storage at 20 to 24 C (room temperature [RTI]) for up to 7 days and cryopreservation for 6 months. Animals-14 mature dogs. Procedure-PC prepared by centrifugation of fresh blood were stored for 7 days at RT with continuous agitation. An aliquot of each was cryopr eserved with 6% dimethyl sulfoxide at -74 C for 6 months. Fresh PC (da y 0) were tested by microbial culture and measurement of platelet coun t, mean platelet volume, pH, glucose and lactate concentrations, lacta te dehydrogenase activity, response to hypotonic stress, and aggregati on. Tests were also performed on PC stored at RT on days 3, 5, and 7, and on the cryopreserved aliquots after thawing. Results-After 7 days at RT, microbial growth was not evident, and decrease in platelet numb er was not significant. On the basis of pH and glucose and lactate con centrations, metabolic activity was maintained throughout RT storage. On the basis of mean platelet volume and lactate dehydrogenase activit y, platelet swelling and membrane damage had occurred. Aggregatory res ponses decreased during RT storage, and platelets recovered poorly fro m hypotonic stress. Af ter cryopreservation for 6 months, microbial gr owth was not evident, but platelet numbers were significantly decrease d. Mean platelet volume and lactate dehydrogenase activity were signif icantly greater, compared with values for day-0 PC, Crypreserved plate lets aggregated poorly and did not respond to hypotonic stress. Conclu sions-Platelet viability and microbiological sterility are retained in canine PC stored for 7 days at RT, but platelet function pregressivel y decreases and day-7 platelets are substantially damaged. Crypreserva tion of PC results in considerable damage, compared with that of PC st ored at RT. Clinical Relevance-Similar to human PC, canine PC stored a t RT for up to 5 days can be recommended for treatment.