MESSENGER-RNA EXPRESSION OF MATRIX METALLOPROTEINASES AND TISSUE INHIBITORS OF METALLOPROTEINASE IN INTERFACE TISSUE AROUND IMPLANTS IN LOOSENING TOTAL HIP-ARTHROPLASTY
N. Ishiguro et al., MESSENGER-RNA EXPRESSION OF MATRIX METALLOPROTEINASES AND TISSUE INHIBITORS OF METALLOPROTEINASE IN INTERFACE TISSUE AROUND IMPLANTS IN LOOSENING TOTAL HIP-ARTHROPLASTY, Journal of biomedical materials research, 32(4), 1996, pp. 611-617
Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloprote
inase (TIMPs) play an important role in tissue destruction and remodel
ing. Nine samples of cement interface tissues from nine patients who h
ad failed cemented total hip arthroplasty (THA) were obtained for revi
sion of THA and analyzed on mRNA expression of MMPs and TIMPs. The pre
operative serial radiographic examinations revealed an apparent clear
zone around all implants. We excluded septic loosening as one of the f
actors affecting THA. Three samples were obtained from three different
sites of the ace-tabular interface tissue in each patient. After extr
action of total RNA from 27 samples, we used the reverse-transcription
al polymerase chain reaction (RT-PCR), mRNA of MMP-1, -2, -3, -9, and
TIMP-1 and -2 was detected in the interface tissue. MMP-10 mRNA was no
t detected, yet MMP-1 and MMP-3 mRNA were commonly observed. TIMP-2 mR
NA was also strongly expressed compared to TIMP-1. It was thus demonst
rated that MMPs and TIMPs were produced locally in the cemented tissue
of THA loosening. These findings may suggest that MMPs and TIMPs expr
essed around the implants play a critical role in the progression of a
septic loosening of THA. (C) 1996 John Wiley & Sons, Inc.