ANALYSIS OF SOLUBLE DIETARY FIBER BY ELECTROPHORESIS AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY IN SOFT DRINKS

A clean-up method using gel permeation chromatography (GPC), and a qua litative analysis involving the electrophoresis of soluble dietary fib ers (SDFs), including Polydextrose(R), Pinefibre(R), Cellace(R), Sunfi ber(R) and guar gum, in soft drinks were both developed. Impurities su ch as sucrose, glucose, fructose, coloring agents and vitamins were cl early eliminated by GPC with Sephadex G-10, with sufficient recoveries , in soft drinks. The solutions treated with GPC were then applied to the electrophoresis with a berate buffer. SDFs showed different migrat ion distances and were detected colorimetrically with l-aminopyridine. The spot of Cellace(R) was grayish green in color, while the other sp ots were pale wine red color. SDFs were also determined by a high perf ormance liquid chromatograph equipped with a refractive index detector and a carbohydrate analysis column.