DISTRIBUTION OF NEURONS IMMUNOREACTIVE FOR PARVALBUMIN AND CALBINDIN IN THE SOMATOSENSORY THALAMUS OF THE RACCOON

The aim of this study was to assess the distribution of neurons immuno reactive for parvalbumin (PV), calbindin (CaBP), glutamic acid decarbo xylase (GAD), and gamma-aminobutyric acid (GABA) in the somatosensory thalamus of the raccoon and to compare these features to those of othe r species, especially primates. Immunohistochemistry was used to study the location of these neurons id the ventroposterior nucleus (VP), ve ntroposterior inferior nucleus (VPI), posterior group of nuclei (Po), and reticular nucleus (Pt). A consistent differential pattern of PV-po sitive (PV+) and CaBP-positive (CaBP+) cells was found in the somatose nsory thalamus. Many PV+ neurons were observed in VP and Rt, but very few were found in VPI or Po. In contrast, CaBP+ neurons were distribut ed throughout VP, VPI, and Po but were very sparse or absent in Rt. In the VP nucleus, PV+ cells were distributed in clusters separated by i nterclusteral regions with a sparse distribution of PV+ cell bodies. T he distributions of PV+ and CaBP+ cells tended to be complementary to each other in VP; regions with a high density of PV+ neurons had a low density of CaBP+ cell bodies. Double-labeling experiments revealed ve ry few neurons in which PV and CaBP immunoreactivities were colocalize d. Cells immunoreactive for GAD or GABA were found in PV-dense cluster s of VP; fewer GABAergic neurons were present in the CaBP-dense interc lusteral regions of VP and in VPI and Po. GAD(+) and GABA(+) neurons w ere most prominently distributed in Rt. We conclude that the distribut ions of PV+ and CaBP+ cell bodies in the raccoon somatosensory thalamu s are very similar to those in primates. The density of GABAergic neur ons in the somatosensory thalamus of the raccoon is less than that in the cat and monkey, but the relative distribution of GABAergic neurons in the different somatosensory nuclei is very similar to that in the cat and monkey. These results are discussed in relation to findings in other species and are related to the functions of lemniscal and nonle mniscal somatosensory pathways. (C) 1997 Wiley-Liss, Inc.