Serratia marcescens has emerged in the last few years as an important
nosocomial pathogen, Many methods for typing this organism have been d
escribed, In this study the random amplified polymorphic DNA-polymeras
e chain reaction (RAPD-PCR) was shown to be a convenient typing method
for S. marcescens. Different combinations of primers previously used
for typing other gram-negative bacilli were assessed. The combination
of primer HLWL-74 and 1254 gave distinguishable patterns for different
serotypes and proved to be the most satisfactory, By applying this co
mbination to 175 isolates of S. marcescens, which could be classified
into 38 groups on the basis of serotyping and phage typing, 73 differe
nt RAPD patterns with good reproducibility were obtained. This is, to
our knowledge, the first application of the method to a large collecti
on of S. marcescens representing a wide range of serotypes.