NAFAZATROM INHIBITS PEROXIDATION OF PHOSPHATIDYLCHOLINE LIPOSOMES, HEART HOMOGENATE AND LOW-DENSITY LIPOPROTEINS

In order to contribute to the understanding of the biological properti es of nafazatrom, an antithrombotic agent (NAF), we studied its effect s on peroxidation of low density lipoproteins (LDL), lipid liposomes, heart homogenate, and its interaction with alpha-tocopherol radical. N AF decreased the FeSO4 and H2O2-induced peroxidation of phosphatidylch oline liposomes and heart homogenate, and it decreased peroxidation of LDL induced by CuSO4 or 2,2'-azobis(2-amidinopropane). The antioxidan t effect of NAF was about 3 times less potent than that of alpha-tocop herol (alpha-TOC) in phosphatidylcholine liposomes, and NAF was about 2-4 times more efficient to decrease peroxidation of LDL than alpha-TO C. Possible interaction of NAF with alpha-tocopherol radical alpha-TR) was studied by EPR spectroscopy. NAF decreased the concentration of a lpha-TR, but it was about 100-times less efficient than vitamin C. Thi s may indicate that NAF does not interfere with alpha-TR formation and /or reduction of alpha-TR in biological system. The obtained results m ay help the explanation of biological effects of NAF.