RELATIONSHIP OF REPAIR AND REPLICATIVE DNA-SYNTHESIS TO CELL-CYCLE INCHINESE-HAMSTER OVARY (CHO-K1) CELLS

Authors
BANFALVI G POIRIER LA MIKHAILOVA M CHOU MW
Citation
G. Banfalvi et al., RELATIONSHIP OF REPAIR AND REPLICATIVE DNA-SYNTHESIS TO CELL-CYCLE INCHINESE-HAMSTER OVARY (CHO-K1) CELLS, DNA and cell biology, 16(10), 1997, pp. 1155-1160
Citations number
31
Categorie Soggetti
Cell Biology",Biology,"Genetics & Heredity
Journal title
DNA and cell biology → ACNP
ISSN journal
10445498
Volume
16
Issue
10
Year of publication
1997
Pages
1155 - 1160
Database
ISI
SICI code
1044-5498(1997)16:10<1155:RORARD>2.0.ZU;2-B
Abstract
To strengthen the causal association between repair and replicative DN A synthesis, we have simultaneously measured the two types of DNA synt hesis in a cell cycle-dependent manner. Synchrony was obtained by coun terflow centrifugal elutriation of logarithmic-phase Chinese hamster o vary (CHO) cells kept in suspension cultures. A comparison of cell cyc le profiles of ATP-dependent replicative and ATP-independent repair sy nthesis in permeable cells shows opposite trends. The rates of repair synthesis and replication are inversely correlated.