Bm. Rosner et al., IN-VITRO STUDIES ON REDUCTIVE VINYL-CHLORIDE DEHALOGENATION BY AN ANAEROBIC MIXED CULTURE, Applied and environmental microbiology, 63(11), 1997, pp. 4139-4144
Reductive dehalogenation of vinyl chloride (VC) was studied in an anae
robic mixed bacterial culture. In growth experiments, ethene formation
from VC increased exponentially at a rate of about 0.019 h(-1). Reduc
tive VC dehalogenation was measured in vitro by using cell extracts of
the mixed culture. The apparent K-m for VC was determined to be about
76 mu M; the V-max was about 28 nmol.min(-1).mg of protein(-1). The V
C-dehalogenating activity was membrane associated. Propyl iodide had a
n inhibitory effect on the VC-dehalogenating activity in the in vitro
assay. However, this inhibition could not be reversed by illumination.
Cell extracts also catalyzed the reductive dehalogenation of cis-1,2-
dichloroethene (cis-DCE) and, at a lower rate, of trichloroethene (TCE
). Tetrachloroethene (PCE) was not transformed. The results indicate t
hat the reductive dehalogenation of VC and cis-DCE described here is d
ifferent from previously reported reductive dehalogenation of PCE and
TCE.