DETECTION OF ACTIVITY RESPONSIBLE FOR INDUCTION OF THE AGROBACTERIUM-TUMEFACIENS VIRULENCE GENES IN BACTERIOLOGICAL AGAR

Authors
LOUBENS I CHILTON WS DION P
Citation
I. Loubens et al., DETECTION OF ACTIVITY RESPONSIBLE FOR INDUCTION OF THE AGROBACTERIUM-TUMEFACIENS VIRULENCE GENES IN BACTERIOLOGICAL AGAR, Applied and environmental microbiology, 63(11), 1997, pp. 4578-4580
Citations number
20
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
63
Issue
11
Year of publication
1997
Pages
4578 - 4580
Database
ISI
SICI code
0099-2240(1997)63:11<4578:DOARFI>2.0.ZU;2-3
Abstract
Agrobacterium tumefaciens C58 grown on acidic medium containing glucos e and solidified with bacteriological agar expressed a virB::lacZ fusi on. No expression of this fusion was observed on a similar medium,whic h was solidified with purified agarose. The fraction from bacteriologi cal agar which was responsible for vir gene induction was extracted wi th methanol and partially purified by preparative thin-layer chromatog raphy.