F-2-ISOPROSTANE AND 4-HYDROXYNONENAL EXCRETION IN HUMAN BILE OF PATIENTS WITH BILIARY-TRACT AND PANCREATIC DISORDERS

Objective: To assess parameters of lipid peroxidation in bile of patie nts with hepatobiliary and pancreatic diseases, Methods: F-2-isoprosta nes (F-2-IPs) and 4-hydroxynonenal (4-HNE) were measured in bile colle cted during 31 ERCP procedures using gas chromatography/mass spectrome try, Results: In 11 subjects with normal ERCP (controls), bile contain ed significant amounts of F-2-IPs (188 +/- 27 pg/ml) and 4-HNE (37.5 /- 8.0 ng/ml), In 10 individuals with bile duct stones, there was a 3- fold increase of F-2-IPs (523 +/- 129 pg/ml; p < 0.05) and a 2.5-fold increase of 4-HNE (89.6 +/- 18.0 ng/ml; p < 0.05), In 10 patients with various pancreatic diseases, bile F-2-IPs were also enhanced (545 +/- 112 pg/ml; p < 0.01), There was no significant change in alpha-tocoph erol, whereas beta-carotene was decreased in biliary tract and pancrea tic diseases (p < 0.05), Results of serum liver tests were normal with the exception of bilirubin, which was increased together with alkalin e phosphatase. Concentrations of total lipids, phospholipids, and chol esterol did not differ significantly between the three groups, Conclus ions: These data provide the first evidence in humans supporting the r ole of oxidative stress in the pathogenesis of biliary and pancreatic disease.