RAPD-PCR UP-PCR RDNA RFLP, RDNA SEQUENCE AND ISOZYME ANALYSES OF FUSARIUM ISOLATES IN FINLAND

Isozyme analysis and both PCR methods with random (RAPD-PCR) and rando m-like (UP-PCR) primers are suitable for intraspecific identification and phylogenetic studies in F. avenaceum and F. oxysporum. RAPD-PCR an d UP-PCR can also be used for identification of Fusarium species. rDNA RFLP and rDNA sequence analyses were more suitable for identification and phylogenetic studies at species level, but their resolution at st rain level was clearly poorer than in RAPD-PCR and UP-PCR analyses.