SUBSTRATE INFLUENCE ON MYCOTOXIN PRODUCTION OF FUSARIUM SPECIES AND THEIR ANALYTICAL DETECTION

Several strains of Fusarium culmorum, F. graminearum, F. moniliforme, F. tricinctum, F. roseum, F. semitectum and F. sporotrichioides were i nvestigated on corn and rice substrate for F-2, zearalenol and T-2 tox in synthesis. Toxin production was confirmed by GLC and estimated by T CLC. Substrate didn't influence significantly recovery of the investig ated mycotoxins in case of rice and corn and was about 70%. Sensitivit y of TLC method is lowest for T-2 (0,05 mu g/spot), detection of F-2 a nd zearalenol could be improved with Sarudi reagent. Toxin synthesis o f individual strains showed depence on substrate.