RAPID THIN-LAYER-CHROMATOGRAPHIC METHODS FOR THE DETERMINATION OF MICROBIOLOGICAL DEGRADATION PRODUCTS OF DEOXYNIVALENOL

The toxic function of deoxynivalenol (DON) is the 12,13-epoxy-group be cause of its potential to inhibit protein biosynthesis. A possible way to detoxify DON is microbiological transformation of the epoxide to a diene. The aim of this work was to develop rapid analytical methods f or monitoring of anaerobic and aerobic microbiological transformation experiments with DON. Thin-layer-chromatography (TLC) is beside high p ressure liquid chromatography (HPLC) and gas chromatography GC known a s a conventional method for the determination of trichothecene mycotox ins. We developed a TLC method with silica gel 60 plates for the deter mination of the aerobic transformation products of DON and a RP-TLC me thod for the determination of anaerobic metabolites. Without the need of any clean-up these methods give extremely rapid results at a very l ow cost.