H. Kobayashi et al., HEMATOLOGIC MALIGNANCIES WITH THE T(10-11)(P13-Q21) HAVE THE SAME MOLECULAR EVENT AND A VARIETY OF MORPHOLOGIC OR IMMUNOLOGICAL PHENOTYPES, Genes, chromosomes & cancer, 20(3), 1997, pp. 253-259
Previous studies described the t(10;11)(p13-14;q14-21) as a recurring
translocation associated with T-cell acute lymphoblastic leukemia (ALL
). This translocation has also been reported in monocytic leukemia or
ALL with a very early pre-B phenotype. However, whether these cytogene
tically similar translocations involve the same molecular breakpoint i
s unknown. Using fluorescence in situ hybridization (FISH) with a seri
es of probes on 11q, we mapped the 11q breakpoint of the U937 cell lin
e, which was derived from a patient with diffuse histiocytic lymphoma
and was shown by FISH to have the t(10;11)(p13-14;q14-21). Subsequentl
y, we identified a yeast artificial chromosome (YAC) clone, y960g8, th
at included the breakpoint on 11q. From this YAC, we isolated a PI clo
ne, P91B1, that was split by the 10;11 translocation. We studied four
patients with a t(10;11), one of whom had acute monocytic leukemia (AM
oL), one had acute lymphoblastic leukemia (ALL), one had lymphoblastic
lymphoma (LBL), and one had granulocytic sarcoma, by using FISH with
y960g8 and P91B1. Y960g8 and P9IBI were split by the translocation in
each patient. We showed that P9IBI included a recently identified gene
, CALM (Clathrin Assembly Lymphoid Myeloid leukemia gene), and that AF
10 was also rearranged in each patient by FISH when we used y807b3, wh
ich contains the AF10 gene. These findings indicate that hematologic m
alignant diseases with fusion of AF10 and CALM show various morphologi
c and immunologic phenotypes, suggesting that this fusion occurs in mu
ltipotential or very early precursor cells. (C) 1997 Wiley-Liss, Inc.