SUPERCRITICAL CARBON-DIOXIDE EXTRACTION OF SARDINE SARDINA-PILCHARDUSOIL

Supercritical fluid extraction was employed to remove lipids from sard ine (Sardina pilchardus) muscle. Extraction experiments using carbon d ioxide under pressures of 12.6 and 18.0 MPa and temperatures of 313 an d 321 K were conducted. Lending of sardine oil in CO2 was found to inc rease with increasing pressure, to diminish with increasing temperatur e and was independent of residence rime. A slight increase in polyunsa turated fatty acid content in the oil extracted by supercritical CO2 w as observed throughout the extraction. Supercritically-extracted sardi ne oil was found to be unstable. Results were compared with those obta ined using the Bligh and Dyer method. (C) 1997 Academic Press Limited.