DIRECT ELECTROPORATION OF CLOSTRIDIAL HYDROGENASE INTO CYANOBACTERIALCELLS

Authors
MIYAKE M ASADA Y
Citation
M. Miyake et Y. Asada, DIRECT ELECTROPORATION OF CLOSTRIDIAL HYDROGENASE INTO CYANOBACTERIALCELLS, Biotechnology techniques, 11(11), 1997, pp. 787-790
Citations number
10
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
11
Issue
11
Year of publication
1997
Pages
787 - 790
Database
ISI
SICI code
0951-208X(1997)11:11<787:DEOCHI>2.0.ZU;2-N
Abstract
Clostridial hydrogenase was introduced into a cyanobacterium, Synechoc occus elongatus, by direct electroporation. The introduced hydrogenase activity was 11 nmol H-2 evolved with reduced Methyl Viologen per mg chlorophyll. The cells with clostridial hydrogenase showed simultaneou s light-dependent evolution of H-2 and O-2. This method will be applic able to assess a variety of hydrogenases for hydrogen production coupl ed with cyanobacterial photosynthesis.