THE CYTOKINE INTERLEUKIN-6 INCREASES EXPRESSION OF THE CELL-ADHESION MOLECULES E-SELECTIN AND VCAM-1 ON ENDOTHELIAL-CELLS IN-VITRO - A ROLEIN PREECLAMPSIA
F. Lyall et al., THE CYTOKINE INTERLEUKIN-6 INCREASES EXPRESSION OF THE CELL-ADHESION MOLECULES E-SELECTIN AND VCAM-1 ON ENDOTHELIAL-CELLS IN-VITRO - A ROLEIN PREECLAMPSIA, Hypertension in pregnancy, 16(3), 1997, pp. 403-415
Objective: To determine whether the previously reported increases in I
nterleukin-6 and cell adhesion molecules in preeclampsia may be linked
, the effects of Interleukin-6 on endothelial cell expression of cell
adhesion molecules, E-Selectin and vascular cell adhesion molecule (VC
AM), were studied in vitro. Methods: Endothelial cells were prepared f
rom umbilical veins and used on the first passage. VCAM and E-Selectin
mRNA were assessed by Northern analysis following incubation of the c
ells with Interleukin-6 (0, 10, 100, 500, or 1000 Units/mL) for 4 h at
37 degrees C. VCAM-1 and E-Selectin proteins were assessed by immunoc
ytochemistry following incubation of the cells with Interleukin-6 (500
Units/mL) for 0, 4, 8, or 24 h at 37 degrees C. Results: Interleukin-
6 resulted in a dose-and time-dependent increase in mRNA and protein l
evels of both E-Selectin and protein. Conclusions: These results sugge
st that Interleukin-6 may, at least in part, be responsible for the in
crease in E-Selectin and VCAM-1 that occur in this disorder.