Vn. Mutafovayambolieva et al., EFFECTS OF NATRIURETIC PEPTIDES AND ENDOTHELINS ON THE NERVE-EVOKED RELEASE OF ADENINE-NUCLEOTIDES AND NUCLEOSIDES IN GUINEA-PIG VAS-DEFERENS, Canadian journal of physiology and pharmacology, 75(9), 1997, pp. 1083-1087
Two members of the natriuretic peptide family (rANF(8-33) and pBNP(1-3
2)) and two members of the endothelin family (ET-1 and ET-2) have been
studied for their effects on the neurogenically induced overflow of a
denosine 5'-triphosphate (ATP), adenosine 5'-diphosphate (ADP), adenos
ine 5'-monophosphate (AMP), and adenosine (ADO) from the isolated guin
ea-pig vas deferens. rANF, pBNP, ET-1, and ET-2 each at 10 nM produced
a significant increase in the evoked overflow of ATP, by 52, 85, 130,
and 11.5%, respectively. None of the peptides altered the overflow of
ADO. ET-1 and ET-2 each caused an increase in the overflow of ADP and
AMP by an amount similar to their effects on ATP overflow, so that th
e ratio ATP:ADP remained 1:1 throughout. Natriuretic peptides, however
, affected the overflow of ADP and AMP to a lesser extent than ATP, re
sulting in an ATP:ADP ratio of 2:1 after rANF and of 1.5:1 after pBNP.
In addition, rANF or pBNP, but not ET-1 or ET-2, inhibited ecto-ATPas
e activity, suggesting that this mechanism may contribute to the facil
itatory effect of the natriuretic peptides on the nerve-evoked overflo
w of ATP in this tissue.