STIMULATING EFFECT OF AN ORAL POLYBACTERIAL IMMUNOMODULATOR ON THE PROLIFERATIVE ACTIVITY OF GUINEA-PIG LYMPHOCYTES

A preparation for the prophylaxis and treatment of inflammations of or al mucosa and parodont Dentavax (D) was investigated in guinea pigs. A nimals were given orally D for 5 consecutive days and a month later th e procedure was repeated. On day 3, 10, 21, and 28 after immunization and reimmunization lymphoproliferative responses to PHA, rIL-2, LPS an d D were measured by the radiometric blast transformation assay in per ipheral blood, spleen, mesenteric lymph nodes (MLN) and Peyer's patche s (PP). ?he percentage of cells entering S and G2/M-phases of cell cyc le was assessed by the flow cytometric DNA analysis. A correlation in proliferative activity of cells after in vitro stimulation with PHA an d LPS has been established by both methods. Peak values of lymphocyte stimulation were found on day 10, especially after the second administ ration of D in all organs tested, mainly in MLNs and spleen. Electron- microscopic studies demonstrated an extensive development of the endop lasmatic reticulum in plasmatic cells from spleen, PPs, mesenteric, br onchial and inguinal lymph nodes. The results obtained may be consider ed a proof of the immunostimulating effect of Dentavax. (C) 1997 Inter national Society for Immunopharmacology.