EVALUATION OF SUSCEPTIBILITY TO OXIDATION OF LINOLEYL DERIVATIVES BY THIN-LAYER CHROMATOGRAPHY WITH FLAME IONIZATION DETECTION

A simple and rapid method for the evaluation of susceptibility to oxid ation in microsamples was developed using a thin-layer chromatographic -flame ionization detection (TLC-FID) system. The procedure was applie d to linoleic acid, methyl linoleate, trilinolein and sucrose octalino leate. Samples of 15 mu g were spotted on Chromarods, subjected to dif ferent temperatures for various times and analysed by TLC-FID. Oxidize d products from linoleyl derivatives were determined from the amounts of unoxidized samples, determined in turn by two approaches, i.e., usi ng calibration graphs or adding squalane an an internal standard.