DETERMINATION OF 1-HYDROXYPYRENE USING HI GH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION

Authors
BAREK J BENCKO V CVACKA J MEJSTRIK V SLAMOVA A SVAGROVA I ZIMA J
Citation
J. Barek et al., DETERMINATION OF 1-HYDROXYPYRENE USING HI GH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION, Chemicke listy, 91(10), 1997, pp. 871-876
Citations number
10
Categorie Soggetti
Chemistry
Journal title
Chemicke listy → ACNP
ISSN journal
00092770
Volume
91
Issue
10
Year of publication
1997
Pages
871 - 876
Database
ISI
SICI code
0009-2770(1997)91:10<871:DO1UHG>2.0.ZU;2-R
Abstract
Optimum conditions are described for HPLC determination of 1-hydroxypy rene with electrochemical detection (ED) giving the detection limit si milar to 1.10(-8) mol.l(-1). This detection limit was compared with th at for UV spectrophotometric detection (similar to 1.10(-7) mol.l(-1)) and fluorimetric detection (similar to 5.10(-10) mol.l(-1)). It was d emonstrated that HPLC-ED can be used for the determination of 1-hydrox ypyrene in urine down to 1.10(-7) mol.l(-1) and thus for biological mo nitoring of human exposure to polycyclic aromatic hydrocarbons.