HIGH-RATE OF INTESTINAL-ABSORPTION OF THE PHOSPHOLIPID ANALOG 1-DODECYL 2-[1-C-14] OCTANAMIDO-SN-2-DEOXY-GLYCERO-3-PHOSPHOCHOLINE IN THE RAT

The phospholipid analogue with two short fatty chains, 1-dodecyl-2-[1- C-14] octanamido-sn-2-deoxy-glycero-3-phosphocholine ([C-14] phospholi pid analogue), with a non-hydrolyzable bond at position 2 of the glyce rol, is an inhibitor of phospholipase A(2). It was obtained after chem ical synthesis and 0.5 mu mol was solubilized in Na+ taurocholate with an equimolar amount of 1-octadecanoyl -[H-3]eicosatetraenoyl-sn-glyce ro-3-phosphocholine which is the current substrate of phospholipases A (2). Both molecules were introduced into the duodenum of rats in order to follow their captations by intestinal mucosa cells for 30, 60 or 9 0 min. The [C-14] phospholipid analogue was poorly split by phospholip ases A(2) (pancreatic juice and intracellular enzymes). It disappeared from the intestinal contents (87% of the dose gone in 90 min) as rapi dly as the tritiated lecithin (81%) but this was later split by the ph ospholipases at a higher rate. (C) 1997 The Italian Pharmacological So ciety.