EFFECT OF CYTOCHALASIN-D ON SYSTEMIC AND LOCAL ANAPHYLAXIS IN A MURINE MODEL

We investigated the effect of cytochalasin D on anaphylaxis. Cytochala sin D dose-dependently inhibited systemic anaphylaxis induced by compo und 48/80 in rats. Especially, cytochalasin D inhibited compound 48/80 -induced systemic anaphylaxis 100% with a dose of 1 mu g g(-1) body we ight (BW). Cytochalasin D significantly inhibited serum histamine leve ls induced by compound 48/80. Cytochalasin D (10(-1) mu g g(-1) BW) al so inhibited local anaphylaxis activated by anti-dinitrophenyl (DNP) I gE to 79.6 +/- 1.8%. Cytochalasin D dose-dependently inhibited histami ne release from the rat peritoneal mast cells activated by compound 48 /80 or anti-DNP IgE. The level of cAMP in rat peritoneal mast cells, w hen cytochalasin D was added, transiently and significantly increased about fourfold compared with that of basal cells. Our studies provide evidence that cytochalasin D will be beneficial in the treatment of an aphylaxis. (C) 1997 The Italian Pharmacological Society.