EFFICIENT IN-SITU REDOX CATALYTIC NAD(P)(-SYNTHESIS USING TRANSITION-METAL COMPLEXES OF 1,10-PHENANTHROLINE-5,6-DIONE AND ITS N-MONOMETHYLATED DERIVATIVE AS CATALYSTS() REGENERATION IN ENZYMATIC)

In comparative studies, we have been able to demonstrate that redox ca talysts based on transition-metal complexes using 1,10-phenanthroline- 5,6-dione as a ligand or based on N-methylated 1,10-phenathroline-5,6- dione acting via hydride ion abstraction are superior to alternative m ethods for the redox catalytic aerobic or indirect electrochemical in situ NAD(P)(+) regeneration in enzymatic syntheses using alcohol dehyd rogenases as production enzymes. Under preparative conditions in the g ram scale we were able to obtain turnover frequencies of up to 130 tur novers per hour with respect to the redox catalyst. These are far larg er than those of the presently most popular regeneration system.