Two strains of parotitis virus were isolated from patients with clinic
al symptoms of the disease in epidemiological screening which was carr
ied out during an outbreak of epidemic parotitis in the village of Kol
tsovo in 1994. The strains were isolated from the saliva of children a
ged 7 and 8 years vaccinated with live parotitis vaccine at the age of
1.5 years. Primers for the genome site coding for the gene F terminal
and the SH gene (a total of 509 n. p.) were estimated and synthesized
and the site was amplified. Electron-microscopic examination of purif
ied virus and Vero cells infected with it and serological tests showed
a similarity of the newly isolated virus with the Anders strain of pa
rotitis virus. The Dragun-1 and Dragun-2 strains of parotitis virus ha
ve been deposited in the collection of viruses at the Vektor State Res
earch Center of Virology and Biotechnology in the village of Koltsovo,
Novosibirsk district.