CHARACTERIZATION OF PAROTITIS VIRUS ISOLA TED IN SIBERIA

Two strains of parotitis virus were isolated from patients with clinic al symptoms of the disease in epidemiological screening which was carr ied out during an outbreak of epidemic parotitis in the village of Kol tsovo in 1994. The strains were isolated from the saliva of children a ged 7 and 8 years vaccinated with live parotitis vaccine at the age of 1.5 years. Primers for the genome site coding for the gene F terminal and the SH gene (a total of 509 n. p.) were estimated and synthesized and the site was amplified. Electron-microscopic examination of purif ied virus and Vero cells infected with it and serological tests showed a similarity of the newly isolated virus with the Anders strain of pa rotitis virus. The Dragun-1 and Dragun-2 strains of parotitis virus ha ve been deposited in the collection of viruses at the Vektor State Res earch Center of Virology and Biotechnology in the village of Koltsovo, Novosibirsk district.