PRODUCTION AND VERIFICATION OF AN ANTI-OVINE IGE MONOCLONAL-ANTIBODY

Recombinant mouse/sheep IgE was used in the production of an anti-IgE monoclonal using conventional hybridoma techniques, The specificity of hybridomas secreting anti sheep IgE monoclonal antibodies was verifie d using a number of assays including competitive ELISAs, ability to in duce mediator release from mast cells, and IgE binding using western b lotting. Immunohistochemical staining demonstrated the binding of puta tive anti-IgE monoclonals to the sheep mast cell surface, The isotype of the antibody was IgG(1):K.