CRYSTALLIZATION AND PRELIMINARY-X-RAY CRYSTALLOGRAPHIC STUDY OF THE RIBOSOMAL-PROTEIN S7 FROM BACILLUS-STEAROTHERMOPHILUS

Authors
HARADA N SANO K KIMURA M HOSAKA H NAKAGAWA A TANAKA I
Citation
N. Harada et al., CRYSTALLIZATION AND PRELIMINARY-X-RAY CRYSTALLOGRAPHIC STUDY OF THE RIBOSOMAL-PROTEIN S7 FROM BACILLUS-STEAROTHERMOPHILUS, Journal of structural biology, 120(1), 1997, pp. 112-114
Citations number
23
Categorie Soggetti
Cell Biology",Biology
Journal title
Journal of structural biology → ACNP
ISSN journal
10478477
Volume
120
Issue
1
Year of publication
1997
Pages
112 - 114
Database
ISI
SICI code
1047-8477(1997)120:1<112:CAPCSO>2.0.ZU;2-U
Abstract
Overproduction and crystallization of Bacillus stearothermophilus ribo somal protein S7 (BstS7), a primary 16S rRNA binding protein and also a translational repressor protein, have been performed to analyze its three-dimensional structure by X-ray crystallography. Ribosomal protei n BstS7 was expressed in the cytoplasmic fraction of the E. coli cells and purified to homogeneity. This recombinant BstS7 was used to produ ce crystals with P2(1) symmetry that diffracted to 2.5 Angstrom resolu tion which are suitable for high-resolution X-ray crystallographic ana lysis. (C) 1997 Academic Press.