GLUCOSE-METABOLISM IN THE ERYTHROCYTES OF THE BUFFALO (BUBALUS-BUBALIS)

(CO2)-C-14 production from [1-C-14] glucose, the rate of glycolysis me asured by the value of lactate production and the activities of variou s enzymes were determined in buffalo erythrocytes. Buffalo red cell gl ycolytic metabolites were estimated and used for the calculation of th e mass action ratios of reactions catalyzed by the glycolytic enzymes of Bubalus bubalis. A comparison of the values of the mass action rati os with the equilibrium constants of the various glycolytic reactions indicate that hexokinase, phosphofructokinase, phosphoglycerate kinase and pyruvate kinase reactions are displaced from equilibrium, suggest ing a regulatory role for each of these enzymes in buffalo erythrocyte glycolysis. (C) 1997 Elsevier Science Inc.