Lung consists of alveoli enclosed by tissue and both structures contri
bute to volume-dependent scattering of light. It is the purpose of thi
s paper to determine the volume-dependent optical properties of lung.
In vivo interstitial fibre measurements of the effective attenuation c
oefficient mu(eff) at 632.8 nm differed during inspiration (mu(eff) =
2.5 +/- 0.5 cm(-1)) from that during expiration (mu(eff) = 3.2 +/- 0.6
cm(-1)). In vitro measurements on a piglet lung insufflated with oxyg
en from 50 to 150 mi showed the effective attenuation coefficient at 6
32.8 nm decreases as a function of oxygen volume in the lung (at 50 mi
mu(eff) = 2.97 +/- 0.11 cm(-1), at 100 mi mu(eff) = 1.50 +/- 0.07 cm(
-1), and at 150 mi mu(eff) = 1.36 +/- 0.15 cm(-1)). This was explained
by combining scattering of alveoli (Mie theory) with optical properti
es of collapsed lung tissue using integrating sphere measurements. The
ory and measured in vitro values showed good agreement (deviation less
than or equal to 15%). Combination of these data yields the absorptio
n coefficient and scattering parameters of lung tissue as a function o
f lung volume. We conclude that the light fluence rate in lung tissue
should be estimated using optical properties that include scattering b
y the alveoli.