MOLECULAR-DETECTION AND CHARACTERIZATION OF YELLOW-FEVER VIRUS IN BLOOD AND LIVER SPECIMENS OF A NON-VACCINATED FATAL HUMAN CASE

Citation
V. Deubel et al., MOLECULAR-DETECTION AND CHARACTERIZATION OF YELLOW-FEVER VIRUS IN BLOOD AND LIVER SPECIMENS OF A NON-VACCINATED FATAL HUMAN CASE, Journal of medical virology, 53(3), 1997, pp. 212-217
Citations number
23
Categorie Soggetti
Virology
Journal title
ISSN journal
01466615
Volume
53
Issue
3
Year of publication
1997
Pages
212 - 217
Database
ISI
SICI code
0146-6615(1997)53:3<212:MACOYV>2.0.ZU;2-T
Abstract
A yellow fever virus of a South American genotype was identified in th e liver and blood samples of a non-vaccinated European patient after h is return from Brazil. ELISA tests were negative for IgG and positive for IgM against yellow fever. Yellow fever proteins in the formalin-fi xed and paraffin-embedded liver biopsy were detected by immunohistoche mical procedures. Viral RNA extracted from the liver tissue was also d etected using an RT-semi-nested PCR procedure and molecular hybridizat ion. Alignment of the sequence obtained from a gene fragment amplified by RT-semi-nested PCR directly from a blood sample with those of Afri can and South American yellow fever virus strains identified a Brazili an topotype as being responsible for the disease. RT-semi-nested PCR m ay be used advantageously for clinical specimens for rapid and specifi c diagnosis, and with archival biopsy material for retrospective studi es. (C) 1997 Wiley-Liss, Inc.