CHEMICAL AND ENZYMATIC-SYNTHESIS OF GLYCOCONJUGATES .2. HIGH-YIELDINGREGIOSELECTIVE SYNTHESIS OF N-ACETYLLACTOSAMINE BY USE OF RECOMBINANTTHERMOPHILIC GLYCOSIDASES LIBRARY

Authors
LI J WANG PG
Citation
J. Li et Pg. Wang, CHEMICAL AND ENZYMATIC-SYNTHESIS OF GLYCOCONJUGATES .2. HIGH-YIELDINGREGIOSELECTIVE SYNTHESIS OF N-ACETYLLACTOSAMINE BY USE OF RECOMBINANTTHERMOPHILIC GLYCOSIDASES LIBRARY, Tetrahedron letters, 38(46), 1997, pp. 7967-7970
Citations number
21
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Tetrahedron letters → ACNP
ISSN journal
00404039
Volume
38
Issue
46
Year of publication
1997
Pages
7967 - 7970
Database
ISI
SICI code
0040-4039(1997)38:46<7967:CAEOG.>2.0.ZU;2-W
Abstract
beta-Galactosidase activities from the recombinant thermophilic CLONEZ YME(TM) glycosidase library were screened at 70 degrees C for catalysi s of a transgalactosylation from o-nitrophenyl-beta-galactopyranoside to N-acetylglucosamine. Three thermophilic glycosidases (Gly001-06, -0 7 and -09) were found to produce predominantly the beta(1-4)-linked is omer, Gal beta(1-4)GlcNAc with up to 61% yield and less than 10% of th e hydrolysis side reaction product. Thus, commercial recombinant therm ophilic enzyme libraries constitute a novel class of biocatalysts for preparative organic synthesis. (C) 1997 Elsevier Science Ltd.