DETERMINATION OF 5-HYDROXY-L-[BETA-C-11]TRYPTOPHAN AND ITS IN VIVO-FORMED RADIOLABELED METABOLITES IN BRAIN-TISSUE USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - A STUDY SUPPORTING RADIOTRACER KINETICS OBTAINED WITH POSITRON-EMISSION-TOMOGRAPHY

Authors
LINDNER KJ HARTVIG P BJURLING P FASTH KJ WESTERBERG G LANGSTROM B
Citation
Kj. Lindner et al., DETERMINATION OF 5-HYDROXY-L-[BETA-C-11]TRYPTOPHAN AND ITS IN VIVO-FORMED RADIOLABELED METABOLITES IN BRAIN-TISSUE USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - A STUDY SUPPORTING RADIOTRACER KINETICS OBTAINED WITH POSITRON-EMISSION-TOMOGRAPHY, Nuclear medicine and biology, 24(8), 1997, pp. 733-738
Citations number
20
Categorie Soggetti
Radiology,Nuclear Medicine & Medical Imaging
Journal title
Nuclear medicine and biology
ISSN journal
09698051 → ACNP
Volume
24
Issue
8
Year of publication
1997
Pages
733 - 738
Database
ISI
SICI code
0969-8051(1997)24:8<733:DO5AII>2.0.ZU;2-B
Abstract
A high performance liquid chromatographic system was developed for sep aration of C-11-labeled 5-hydroxy-L-tryptophan ([C-11]HTP) and in vivo formed radiolabeled metabolites in rat brain tissue. Analysis of brai n homogenate revealed that the main part of the radioactivity was asso ciated with C-11-labeled 5-hydroxyindole-3-acetic acid after intraveno us injection of [C-11]HTP to the rat, The serotonin synthesis rate in the brain was calculated and closely correlated to the serotonin synth esis rate in monkey and human measured using positron emission tomogra phy. (C) 1997 Elsevier Science Inc.