Kl. Payne et al., MODULATION OF T-3-INDUCED SEX HORMONE-BINDING GLOBULIN SECRETION BY HUMAN HEPATOBLASTOMA CELLS, European journal of endocrinology, 137(4), 1997, pp. 415-420
We have shown previously that tri-iodothyronine (T-3)-induced sex horm
one-binding globulin (SHBG) secretion by the human hepatoblastoma cell
line, HepG2, can be modulated by retinoids. We have now used this mod
el to study a range of other compounds that are known to influence T-3
responsiveness in various cell systems. HepG2 cells were incubated fo
r 4 days in serum-free medium containing T-3, together with insulin, d
examethasone, phorbol myristate (PMA), sodium butyrate or estradiol. T
-3 (10 nmol/l) alone induced a concentration of SHBG secreted by HepG2
cells that was 187+/-20% (mean+/-S.D., n=9) of control. Insulin (100
nmol/l) reduced basal SHBG secretion from 24.7+/-5.2 nmol/l to 16.1+/-
1.7 nmol/1 (P<0.01). This effect was dose responsive, half-maximal at
3.4+/-3.0 nmol/l (approximate to 600 mU/l) and maximal with 100 nmol/l
insulin. Co-incubating 0-10 nmol/1 T-3 with 100 nmol/1 insulin result
ed in a downward shift in the dose-response curve without a change in
the half-maximal response to T-3. Conversely, 0-100 nmol/l insulin red
uced SHBG production induced by 10 nmol/l T-3. In contrast, while dexa
methasone alone was without effect on SHBG secretion, 100 nmol/l dexam
ethasone induced a shift to the left in half-maximal T-3 stimulation f
rom 0.37 nmol/l to 0.10 nmol/l. The effect of PMA on SHBG secretion wa
s reminiscent of the previously observed retinoid effect. PMA 100 nmol
/l abolished maximal T-3 stimulation. This effect was dose responsive,
with a threshold at 1 nmol/l PMA. Sodium butyrate, up to 1 nmol/l was
without effect; with greater concentrations, SHBG secretion was reduc
ed. T-3 responsiveness was virtually abolished by 3 mmol/l sodium buty
rate; higher concentrations were cytotoxic and secretion was reduced t
o less than 20% of basal. Lack of an effect of estradiol on SHBG secre
tion by HepG2 cells was confirmed. These studies suggest that T-3-indu
ced SHBG secretion by HepG2 cells is independently influenced by insul
in, potentiated by dexamethasone, and modulated by PMA. Detailed molec
ular analysis of this model will increase our understanding of the mec
hanism of action of T-3, specifically in human liver cells.