ISOLATION AND SEQUENCING OF PORCINE FAST SKELETAL-MUSCLE ALKALI MYOSIN LIGHT-CHAIN-3 CDNA

Authors
DAVOLI R FONTANESI L COSTOSI E ZAMBONELLI P RUSSO V
Citation
R. Davoli et al., ISOLATION AND SEQUENCING OF PORCINE FAST SKELETAL-MUSCLE ALKALI MYOSIN LIGHT-CHAIN-3 CDNA, Animal biotechnology, 8(2), 1997, pp. 179-185
Citations number
16
Categorie Soggetti
Agriculture Dairy & AnumalScience","Biothechnology & Applied Migrobiology
Journal title
Animal biotechnology → ACNP
ISSN journal
10495398
Volume
8
Issue
2
Year of publication
1997
Pages
179 - 185
Database
ISI
SICI code
1049-5398(1997)8:2<179:IASOPF>2.0.ZU;2-O
Abstract
A cDNA coding for alkali myosin light chain 3 (MLC3(F)) was isolated f rom a porcine skeletal muscle library. This clone has an insert of 859 bp encompassing the complete CDS (coding sequence) plus the 5' and 3' untranslated regions. Computer analysis showed that porcine MLC3, cDN A is highly homologous to the corresponding cDNAs of human, rabbit and rat. Moreover, Northern analysis showed the presence of two bands tha t represent the mature mRNAs of the MLClF and MLC3(F) isoforms accordi ng to data observed in other species.