C-type inactivation of Shaker potassium channels involves entry into a
state (or states) in which the inactivated channels appear nonconduct
ing in physiological solutions. However, when Shaker channels, from wh
ich fast N-type inactivation has been removed by NH2-terminal deletion
s, are expressed in Xenopus oocytes and evaluated in inside-out patche
s, complete removal of K+ ions from the internal solution exposes cond
uction of Na+ and Li+ in C-type inactivated conformational states. The
present paper uses this observation to investigate the properties of
ion conduction through C-type inactivated channel states, and demonstr
ates that both activation and deactivation can occur in C-type states,
although with slower than normal kinetics. Channels in the C-type sta
tes appear ''inactivated'' (i.e., nonconducting) in physiological solu
tions due to the summation of two separate effects: first, internal K ions prevent Na+ ions from permeating through the channel; second, C-
type inactivation greatly reduces the permeability of K+ relative to t
he permeability of Na+, thus altering the ion selectivity of the chann
el.