AN IMPROVED HPLC METHOD TO PURIFY ERYTHROCYTE CHOLESTEROL FOR ESTIMATION OF IN-VIVO CHOLESTEROL-SYNTHESIS USING THE DEUTERIUM METHOD

Authors
WONG WW HACHEY DL CLARKE LL ZHANG S LLAURADOR M POND WG
Citation
Ww. Wong et al., AN IMPROVED HPLC METHOD TO PURIFY ERYTHROCYTE CHOLESTEROL FOR ESTIMATION OF IN-VIVO CHOLESTEROL-SYNTHESIS USING THE DEUTERIUM METHOD, Applied radiation and isotopes, 45(4), 1994, pp. 529-533
Citations number
3
Categorie Soggetti
Nuclear Sciences & Tecnology","Radiology,Nuclear Medicine & Medical Imaging
Journal title
Applied radiation and isotopes
ISSN journal
09698043 → ACNP
Volume
45
Issue
4
Year of publication
1994
Pages
529 - 533
Database
ISI
SICI code
0969-8043(1994)45:4<529:AIHMTP>2.0.ZU;2-W
Abstract
We report on an improved HPLC method to purify erythrocyte cholesterol for use in measuring in vivo cholesterol synthesis from (H2O)-H-2. Th e new procedure uses a smaller compression cartridge column at a flow rate of 3 mL/min. The new method reduces by almost tenfold the amount of solvent required to maintain the analytical purity of the isolated cholesterol.