ACTIN ISOFORMS IN NONINFECTED ROOTS AND SYMBIOTIC ROOT-NODULES OF PHASEOLUS-VULGARIS L

The present report describes an initial characterization of actin from non-infected roots and symbiotic nodules of Phaseolus vulgaris L. cv. Negro damapa. Using anti-actin monoclonal antibodies, a 42-kDa polype ptide was identified in plant extracts. After two-dimensional SDS-PAGE analysis and Western blotting of actin fractions enriched using dieth ylaminoethyl-resin, the presence of one major isoform of actin in symb iotic nodules and two main isoforms in non-infected roots was revealed . Possible implications of this finding are discussed.