FERTILE SOMATIC HYBRIDS BETWEEN BRASSICA-NAPUS AND ARABIDOPSIS-THALIANA

Fertile somatic hybrids between Brassica napus and Arabidopsis thalian a were produced by protoplast fusion and enriched by flow sorting. Iso enzyme analysis and hybridization of repetitive DNA sequences from the two parental species to total DNA from the hybrids were used to verif y hybrid characteristics of the nuclear genomes. Of 29 shoots, each fr om a different callus, 25 had a hybrid pattern for at least one of the markers investigated. Analysis of chromosome number revealed that 11 out of 18 hybrids had the sum of the two parental chromosome numbers. Organelle analysis of 18 hybrids showed that ten hybrids had mitochond rial (mt) restriction patterns identical to those of B. napus, while e ight displayed a rearranged mtDNA restriction fragment pattern. B. nap us chloroplasts (cp) were favoured in the hybrids, and out of 21 analy sed hybrids only one plant had A. thaliana cp. Most of the hybrids had intermediate morphological features compared with those of the parent al species and 14 out of the 19 tested hybrids were female fertile and set seed after backcrossing to B. napus. Of the female-fertile hybrid s, two were also self-fertile.