STABILIZATION OF POTATO-TUBER LIPOXYGENASE ON TALC

Potato tuber lipoxygenase, like many plant enzymes is a very labile pr otein and can lose activity during or after the purification procedure . In order to overcome this problem, we immobilized potato tuber lipox ygenase by adsorption on talc, a silicate support. Lipoxygenase adsorp tion greatly increased the long-term stability of the enzyme without m odifying enzyme specificity. Moreover, adsorption on talc could be use d to purify labile enzymes whose enzymatic activity decreases rapidly during purification stages. A multi-step reaction to produce large qua ntities (around 100 mg) of fatty acid hydroperoxides without enzyme in activation was developed.