CHARACTERIZATION OF 1,1,1,2-TETRAFLUOROETHANE AND CARBON-DIOXIDE BINARY MOBILE PHASES WITH POLYMERIC COLUMNS USING LINEAR SOLVATION ENERGY RELATIONSHIPS

In this study, the chromatographic properties of binary carbon dioxide /1,1,1,2-tetrafluoroethane (HFC-134a) mobile phases on a polymeric sta tionary phase are examined, Retention of various analytes, as a functi on of mobile phase composition, is dependent upon the physicochemical properties of the analyte. The specific intermolecular interactions be tween various solutes and the mobile and stationary phases responsible for the retention behavior are probed using linear solvation energy r elationships, Using this methodology, it is apparent that solute hydro gen bond donating ability is the dominant factor dictating retention i n these systems, Other solute properties, such as dipolarity/polarizab ility, hydrogen bond accepting ability and excess molar refraction, ha ve much smaller effects on retention.