LOCALIZATION OF GLYCOCONJUGATES AND GLYCOGEN IN YEAST-LIKE CELLS AND PROTOPLASTS OF PARACOCCIDIOIDES-BRASILIENSIS

The presence and localization of storage polysaccharides and of polysa ccharides as cell structure constituents of Paracoccidioides brasilien sis yeast-like cells and protoplasts were studied using the periodic a cid-thiocarbohydrazide-silver proteinate (PATAg) technique. Yeast-like cells presented glycogen particles in the form of rosettes, which wer e mostly concentrated in regions of the cytoplasm. Cells in the buddin g process presented small amounts of glycogen in their matrix. The int racellular membranes and the attachment of the mother cell to the bud were clearly labelled by silver, demonstrating a large amount of glyco conjugates. The protoplasts presented a small amount of glycogen in th eir cytoplasm, a reduction probably due to the enzymatic treatment to which the cells were submitted.