Gas. Passos et al., ECORI RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM OF THE HUMAN-IMMUNOGLOBULIN VARIABLE LAMBDA-8 (IGLV8) SUBGROUP REVEALS A GENE FAMILY, Human immunology, 55(2), 1997, pp. 96-102
The human immunoglobulin lambda locus (IGL) maps on chromosome 22q11.1
-q11.2 and directs che synthesis of lambda-type Ig light chains. This
locus is formed by three gene clusters (VA, VB and VC) that encompass
the variable coding genes and the J-C cluster plus the joining segment
s and the constant genes. Recently the variable lambda gene clusters w
ere mapped by the contig methodology which located all the known funct
ional v-lambda genes and pseudogenes. The 30 functional v-lambda genes
described so far were subgrouped into ten families (V lambda I to V l
ambda X), but RFLP studies have estimated that the germline repertoire
contains about 70 genes. Based on sequence comparisons, we defined sp
ecific oligonucleotide primers for the unique IGLV8S1 gene described.
The cloned 244 bp product obtained from genomic DNA with these primers
was sequenced and used as probe in Southern hybridization EcoRI RFLP
analysis of Brazilian people. We detected the IGLV8S1 gene in a 3.7 kb
EcoRI restriction fragment present in all the individuals analyzed, i
n agreement with the physical map of the IGL locus. Moreover, we detec
ted an 8.0 kb EcoRI monomorphic fragment and a 6.0 kb EcoRI polymorphi
c fragment. These data suggest that the IGLV8 subgroup is a gene famil
y. (C) American Society for Histocompatibility and Immunogenetics, 199
7. Published by Elsevier Science Inc.