ECORI RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM OF THE HUMAN-IMMUNOGLOBULIN VARIABLE LAMBDA-8 (IGLV8) SUBGROUP REVEALS A GENE FAMILY

Citation
Gas. Passos et al., ECORI RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM OF THE HUMAN-IMMUNOGLOBULIN VARIABLE LAMBDA-8 (IGLV8) SUBGROUP REVEALS A GENE FAMILY, Human immunology, 55(2), 1997, pp. 96-102
Citations number
34
Categorie Soggetti
Immunology
Journal title
ISSN journal
01988859
Volume
55
Issue
2
Year of publication
1997
Pages
96 - 102
Database
ISI
SICI code
0198-8859(1997)55:2<96:EROTH>2.0.ZU;2-D
Abstract
The human immunoglobulin lambda locus (IGL) maps on chromosome 22q11.1 -q11.2 and directs che synthesis of lambda-type Ig light chains. This locus is formed by three gene clusters (VA, VB and VC) that encompass the variable coding genes and the J-C cluster plus the joining segment s and the constant genes. Recently the variable lambda gene clusters w ere mapped by the contig methodology which located all the known funct ional v-lambda genes and pseudogenes. The 30 functional v-lambda genes described so far were subgrouped into ten families (V lambda I to V l ambda X), but RFLP studies have estimated that the germline repertoire contains about 70 genes. Based on sequence comparisons, we defined sp ecific oligonucleotide primers for the unique IGLV8S1 gene described. The cloned 244 bp product obtained from genomic DNA with these primers was sequenced and used as probe in Southern hybridization EcoRI RFLP analysis of Brazilian people. We detected the IGLV8S1 gene in a 3.7 kb EcoRI restriction fragment present in all the individuals analyzed, i n agreement with the physical map of the IGL locus. Moreover, we detec ted an 8.0 kb EcoRI monomorphic fragment and a 6.0 kb EcoRI polymorphi c fragment. These data suggest that the IGLV8 subgroup is a gene famil y. (C) American Society for Histocompatibility and Immunogenetics, 199 7. Published by Elsevier Science Inc.