NITROSO-R SALT AS A SENSITIVE SPECTROPHOTOMETRIC REAGENT FOR THE DETERMINATION OF VITAMIN-C

An analytical procedure based on the reaction of nitroso-R salt with r educed iron(II) is developed for the analysis of ascorbic acid. The ab sorbance of the resulting green colored complex is measured at 705 nm. Beer's law is obeyed over the rang 0.2-3.0 mu g ml(-1) ascorbic acid with a molar absorptivity of 4.5 x 10(4) 1 mol(-1) cm(-1). The substan ces commonly found with vitamin C formulations are tolerated. Some of the marketed products of ascorbic acid were analyzed by the proposed m ethod with satisfactory results.