MOLECULAR-CLONING AND CHARACTERIZATION OF A HUMAN BRAIN RYANODINE RECEPTOR

Authors
NAKASHIMA Y NISHIMURA S MAEDA A BARSOUMIAN EL HAKAMATA Y NAKAI J ALLEN PD IMOTO K KITA T
Citation
Y. Nakashima et al., MOLECULAR-CLONING AND CHARACTERIZATION OF A HUMAN BRAIN RYANODINE RECEPTOR, FEBS letters, 417(1), 1997, pp. 157-162
Citations number
41
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
417
Issue
1
Year of publication
1997
Pages
157 - 162
Database
ISI
SICI code
0014-5793(1997)417:1<157:MACOAH>2.0.ZU;2-V
Abstract
We have cloned and sequenced the cDNA of the human brain ryanodine rec eptor (RyR3), which is composed of 4866 amino acids and shares charact eristic structural features with the rabbit RyR3. Northern blot analys is shows that the human RyR3 mRNA is abundantly expressed in hippocamp us, caudate nucleus and amygdala as well as in skeletal muscle. The hu man RyR3 mRNA is also detected in several cell lines derived from huma n brain tumors. Functional expression of RyR3 and a chimeric RyR sugge sts that RyR3 forms a calcium-release channel with a very low Ca2+ sen sitivity. (C) 1997 Federation of European Biochemical Societies.