Ak. Srivastav et al., VITAMIN-D-3-INDUCED CALCEMIC AND PHOSPHATEMIC RESPONSES IN THE FRESH-WATER MUD EEL AMPHIPNOUS-CUCHIA MAINTAINED IN DIFFERENT CALCIUM ENVIRONMENTS, Brazilian journal of medical and biological research, 30(11), 1997, pp. 1343-1348
Vitamin D-3 (100 ng 100 g body weight(-1) day(-1)) was administered in
traperitoneally (ip) to the freshwater mud eel Amphipnous cuchia kept
in artificial freshwater, calcium-free freshwater, low-calcium freshwa
ter (0.2 mmol/l CaCl2) or calcium-rich freshwater (13.4 mmol/l CaCl2)
for 15 days. Analyses of serum calcium and phosphate levels were perfo
rmed on days 1, 3, 5, 10 and 15 after the beginning of the experiment
(six eels from each group at each interval). Administration of vitamin
D-3 elevated the serum calcium [maximum elevation occurred at day 10
in artificial freshwater (vehicle: 10.55 +/- 0.298, vitamin D: 13.90 /- 0.324), low-calcium freshwater(vehicle: 11.17 +/- 0.220, vitamin D:
12.98 +/- 0.297) and calcium-rich freshwater (vehicle: 11.24 +/- 0.37
3, vitamin D: 14.24 +/- 0.208) whereas it occurred at day 5 (vehicle:
8.42 +/- 0.253, vitamin D: 11.07 +/- 0.328) in calcium-free freshwater
] and phosphate levels [maximum elevation at day 15 in artificial fres
hwater (vehicle: 4.39 +/- 0.105, vitamin D: 5.37 +/- 0.121), calcium-f
ree freshwater (vehicle: 4.25 +/- 0.193, vitamin D: 5.12 +/- 0.181), l
ow-calcium freshwater (vehicle: 3.93 +/- 0.199, vitamin D: 5.28 +/- 0.
164) and calcium-rich freshwater (vehicle: 3.77 +/- 0.125, vitamin D:
5.46 +/- 0.151)] of the fish maintained in the above mentioned environ
mental media, but the responses were more pronounced in the fish kept
in calcium-rich media.