TRIGLYCERIDE SYNTHESIS AND SECRETION AND LIPOGENESIS IMPLICATED GENE-EXPRESSION IN THE CHICKEN HEPATOCARCINOMA CELL-LINE LMH

Avian lipogenesis was studied in the chicken hepatocarcinoma LMH cell line, The differentiated and lipogenic status of these cells was evide nced by the presence of the albumin mRNA as well as of some mRNA codin g for enzymes involved in lipogenesis (acetyl-CoA carboxylase, fatty a cid synthase, Delta 9 desaturase) and for apoproteins (apoprotein B an d A1). These results were further confirmed by the analysis of triglyc eride synthesis and secretion rates in growing cells, A time course an alysis showed that triglyceride metabolism was affected by cell densit y. Hormone responsiveness of triglyceride production was also analyzed , Insulin, triiodothyronine and glucagon to a lesser extent were shown to regulate lipogenesis of LMH cells, The results were compared with those obtained in primary cultures of chicken hepatocytes.