RAT ALVEOLAR MACROPHAGES EXPRESS PREPROTACHYKININ GENE-I MESSENGER-RNA-ENCODING TACHYKININS

Although the tachykinins substance P (SP) and neurokinin A have been l argely localized to neurons, eosinophils have also been shown to expre ss these peptides. Our aim was to determine whether rat alveolar macro phages (AM) express preprotachykinin gene-I (PPT-I) mRNA that encodes these tachykinins and to examine expression during inflammation. PPT-I mRNA was detected by reverse transcription (RT)polymerase chain react ion (PCR) in AM and brain (control) but not in peritoneal macrophages. Northern analysis showed that PPT-I mRNA was induced two- to fourfold by in vivo treatment of rats with intratracheal lipopolysaccharide (L PS) and in vitro after 4 h of exposure to LPS. This increase was inhib ited by dexamethasone. In situ RT-PCR and immunocytochemistry further confirmed that AM express PPT-I mRNA and SP-like immunoreactivity, res pectively, which was enhanced by LPS treatment. A 1.3-kb transcript co nsistent with PPT-I mRNA was detected by Northern analysis of bronchoa lveolar lavage neutrophils. Therefore, rat AM express PPT-I mRNA that is upregulated in AM by LPS and is attenuated by dexamethasone. PPT-I mRNA was also detected in lung neutrophils.